5D0A

Crystal structure of epoxyqueuosine reductase with cleaved RNA stem loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	90.587, 111.118, 96.008
Unit cell angles	90.00, 104.90, 90.00

Refinement procedure

Resolution	46.390 - 2.100
R-factor	0.186
Rwork	0.184
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d08
RMSD bond length	0.009
RMSD bond angle	1.088
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge		0.394
Number of reflections	104237
<I/σ(I)>	5.8
Completeness [%]	97.4	89.3
Redundancy	3.4	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	(0.1 M acetate, 0.4 M (NH4)H2PO4, 12% (w/v) PEG 3350