5CZS
Neisseria meningitidis 3 dexy-D-arabino-heptulosonate 7-phosphate synthase Glu98Ala variant regulated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2014-10-23 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.322, 142.326, 74.591 |
| Unit cell angles | 90.00, 96.84, 90.00 |
Refinement procedure
| Resolution | 74.060 - 2.420 |
| R-factor | 0.19458 |
| Rwork | 0.191 |
| R-free | 0.24569 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hsn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.382 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.060 | 2.470 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.156 | 0.906 |
| Number of reflections | 57783 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 99.8 | 97.1 |
| Redundancy | 7.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 296 | 0.1M Tris HCl (pH 7.3), 0.2 M trimethyl-amino-N-oxide (TMAO), 0.4 mM MnSO4 and PEG 2000MME |
