5CYN
JC Virus large T-antigen origin binding domain F258L mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | I 41 |
Unit cell lengths | 103.540, 103.540, 34.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
R-factor | 0.19682 |
Rwork | 0.195 |
R-free | 0.22747 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lif |
RMSD bond length | 0.009 |
RMSD bond angle | 1.190 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.120 | 0.660 |
Number of reflections | 5163 | |
<I/σ(I)> | 37.4 | 2.7 |
Completeness [%] | 99.6 | 96.6 |
Redundancy | 15.4 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris pH 8.5, 25-35% Peg 6000 |