5CWS
Crystal structure of the intact Chaetomium thermophilum Nsp1-Nup49-Nup57 channel nucleoporin heterotrimer bound to its Nic96 nuclear pore complex attachment site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1400 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 123.180, 162.820, 212.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.666 - 3.770 |
| R-factor | 0.2309 |
| Rwork | 0.229 |
| R-free | 0.26540 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.678 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 3.770 | 3.770 |
| Rmerge | 0.204 | |
| Number of reflections | 43896 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 99.9 | |
| Redundancy | 40.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 294 | 0.1M TRIS, pH 8.7 5.7 % (w/v) PEG 20,000 1 % (v/v) 1-propanol |
