5CVO

WDR48:USP46~ubiquitin ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	211.342, 103.764, 190.954
Unit cell angles	90.00, 119.03, 90.00

Refinement procedure

Resolution	47.704 - 3.885
R-factor	0.2247
Rwork	0.222
R-free	0.27530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cvn
RMSD bond length	0.005
RMSD bond angle	1.304
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.880
Rmerge	0.202
Number of reflections	28480
<I/σ(I)>	8.2
Completeness [%]	95.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	15% MPD, 0.1 M NaCl, 0.1 M HEPES, pH 7.0