5CVM

USP46~ubiquitin BEA covalent complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	C 2 2 21
Unit cell lengths	90.613, 105.120, 134.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.820 - 1.900
R-factor	0.1767
Rwork	0.176
R-free	0.19720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.820	50.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge		0.030	0.805
Number of reflections	50336
Completeness [%]		99.5	97.6
Redundancy		5.9	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	3.6 M sodium formate