5CUS
Crystal Structure of sErbB3-Fab3379 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-12-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.378, 127.141, 138.078 |
| Unit cell angles | 87.10, 85.54, 89.92 |
Refinement procedure
| Resolution | 69.390 - 3.200 |
| R-factor | 0.2535 |
| Rwork | 0.252 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4LEO and 3H42 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.568 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.390 | 3.314 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.102 | 0.321 |
| Number of reflections | 92683 | |
| <I/σ(I)> | 7.45 | 2.62 |
| Completeness [%] | 98.3 | 98.25 |
| Redundancy | 1.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | PEG 3350, Ammonium Citrate, Sodium Thiocyanate |
