5CTT

Crystal structure of human SART3/TIP110 NLS-mouse importin alpha complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	79.149, 89.992, 97.818
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.765 - 1.700
R-factor	0.1918
Rwork	0.191
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ial
RMSD bond length	0.007
RMSD bond angle	1.094
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.078	0.453
Number of reflections	77648
<I/σ(I)>	19.8	1.96
Completeness [%]	99.0	97.4
Redundancy	2.8	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM HEPES, pH 6.6, 1.6M sodium citrate, 10mM DTT