5COV

Structure and mechanism of a eukaryal nick-sealing RNA ligase K170M+Mn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.97920
Spacegroup name	P 32
Unit cell lengths	55.479, 55.479, 98.551
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.740 - 2.200
R-factor	0.19442
Rwork	0.191
R-free	0.26711
RMSD bond length	0.012
RMSD bond angle	1.610
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200	2.200
Rmerge		0.290
Number of reflections	16304
<I/σ(I)>	22.1	2
Completeness [%]	95.0	84.6
Redundancy	2.3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M HEPES, 30% PEG 6000, 1 mM Mn