5COU

Structure and mechanism of a eukaryal nick-sealing RNA ligase K170M+ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-14
Detector	PSI PILATUS 6M
Wavelength(s)	0.97921
Spacegroup name	P 32
Unit cell lengths	54.969, 54.969, 100.308
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.100 - 1.900
R-factor	0.17952
Rwork	0.176
R-free	0.24672
RMSD bond length	0.019
RMSD bond angle	2.081
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.950
High resolution limit [Å]	1.900	1.900
Rmerge		0.437
Number of reflections	26788
<I/σ(I)>	27.1	3
Completeness [%]	100.0	99.9
Redundancy	7.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M HEPES, 30% PEG 6000, 2 mM ATP