5CNJ
mGlur2 with glutamate analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-18 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 80.391, 155.087, 92.714 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.040 - 2.650 |
R-factor | 0.1945 |
Rwork | 0.193 |
R-free | 0.24120 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.040 |
High resolution limit [Å] | 2.650 |
Number of reflections | 33677 |
<I/σ(I)> | 7.6 |
Completeness [%] | 97.9 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 100mM Hepes pH 7 + 20% PEG 3350 + 200mM Magnesium Chloride |