5CMS
Structural Insights into the Mechanism of Escherichia coli Ymdb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 290.019, 290.019, 114.324 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.900 - 2.980 |
| R-factor | 0.2231 |
| Rwork | 0.222 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1spv |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.841 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.980 | 3.000 |
| Rmerge | 0.166 | 0.783 |
| Number of reflections | 109753 | |
| <I/σ(I)> | 14 | 2.8 |
| Completeness [%] | 98.1 | 100 |
| Redundancy | 10.6 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 285 | 2 M ammonium sulfate, 0.1 M BIS-TRIS |
