5CMN
FLRT3 LRR domain in complex with LPHN3 Olfactomedin domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2014-12-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.00883 |
| Spacegroup name | P 43 |
| Unit cell lengths | 102.268, 102.268, 419.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.196 - 3.605 |
| R-factor | 0.1987 |
| Rwork | 0.195 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wxq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.938 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 3.660 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.229 | 0.568 |
| Number of reflections | 45263 | |
| <I/σ(I)> | 6.031 | 1.347 |
| Completeness [%] | 92.0 | 60.6 |
| Redundancy | 3.6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 295 | 10% (w/v) PEG 3000, 100mM MES pH6.0, 200mM Lithium sulfate |
