5CMM
Crystal structure of the GluK2EM LBD dimer assembly complex with 2S,4R-4-methylglutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-01 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.000 |
Spacegroup name | P 61 |
Unit cell lengths | 52.308, 52.308, 170.923 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.287 - 1.271 |
R-factor | 0.1441 |
Rwork | 0.143 |
R-free | 0.16320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s50 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.236 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.290 |
High resolution limit [Å] | 1.270 | 1.270 |
Rmerge | 0.043 | 0.335 |
Number of reflections | 68757 | |
<I/σ(I)> | 45.64 | 3.8 |
Completeness [%] | 99.4 | 95.2 |
Redundancy | 7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | Reservoir: 18% PEG 4K Protein buffer: 20 mM NaCl, 5 mM 2S,4R-4-methylglutamate, 1 mM EDTA, 2 mM TRIS pH 8 |