5CM8
Structural Basis for the Selectivity of Guanine Nucleotide Exchange Factors for the small G-protein Ral
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95372 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 111.110, 98.490, 71.320 |
Unit cell angles | 90.00, 91.61, 90.00 |
Refinement procedure
Resolution | 44.420 - 2.600 |
R-factor | 0.26209 |
Rwork | 0.259 |
R-free | 0.32188 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4JGW as poly Ala model |
RMSD bond length | 0.006 |
RMSD bond angle | 0.927 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.096 | |
Number of reflections | 23162 | |
<I/σ(I)> | 15.81 | 3.21 |
Completeness [%] | 97.7 | 99.6 |
Redundancy | 3.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.45 | 289 | 15% PEG 3350, 200 mM ammonium acetate, 100 mM Bis-Tris propane |