5CIY
Structural basis of the recognition of H3K36me3 by DNMT3B PWWP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 95.335, 95.335, 314.688 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.554 - 1.594 |
| R-factor | 0.1664 |
| Rwork | 0.165 |
| R-free | 0.18830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9mht |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.161 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 | |
| High resolution limit [Å] | 1.590 | 4.760 | 1.590 |
| Rmerge | 0.045 | 0.024 | 0.694 |
| Rmeas | 0.049 | 0.026 | 0.808 |
| Total number of observations | 477758 | ||
| Number of reflections | 72764 | 2864 | 10955 |
| <I/σ(I)> | 25.05 | 53.13 | 2.08 |
| Completeness [%] | 98.5 | 96.4 | 92.7 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | AMMONIUM SULFATE 1.8 M, CITRATE 50mM |
