5CHK
Crystal structure of avidin - HABA complex (hexagonal crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977609 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 61.310, 61.310, 120.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.180 - 2.200 |
| R-factor | 0.16401 |
| Rwork | 0.161 |
| R-free | 0.22746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vyo |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.003 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 7329 |
| <I/σ(I)> | 31.92 |
| Completeness [%] | 100.0 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292.15 | 0.1M sodium citrate tribasic dehydrate pH 4.5, 24% w/v polyethylene glycol 1000 |
