5CHG

Human DNA polymerase lambda L431A mutant- MgdGTP binary and complex with 6 paired DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	95
Detector technology	CCD
Collection date	2013-06-29
Detector	RAYONIX MX300HE
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	83.893, 83.893, 398.646
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.892 - 2.900
R-factor	0.2093
Rwork	0.205
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xsl
RMSD bond length	0.005
RMSD bond angle	0.860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.000
High resolution limit [Å]	2.900	6.230	2.900
Rmerge	0.170	0.046	0.879
Total number of observations	272760
Number of reflections	19664
<I/σ(I)>	7
Completeness [%]	100.0	99.6	100
Redundancy	13.9	12.2	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M Lithium Sulfate monohydrate, 0.1M HEPES-Sodium, 0.1M Potassium Sodium Tartrate