5CHF

Crystal structure of murine ISG15 in space group P21212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	57.990, 168.290, 85.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.966 - 2.300
R-factor	0.2105
Rwork	0.208
R-free	0.26570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z2m
RMSD bond length	0.006
RMSD bond angle	1.078
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.400
High resolution limit [Å]	2.300	8.000	2.300
Rmerge	0.182	0.030	1.762
Rmeas	0.197	0.033	1.919
Total number of observations	267134
Number of reflections	37611	1001	4314
<I/σ(I)>	10.91	41.54	1.04
Completeness [%]	99.0	97.8	96.2
Redundancy	7.1		6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.7 M (NH4)2 SO4, 0.06 mM ZnSO4