5CGP
Selective pharmacological inhibition of the CREB binding protein bromodomain regulates inflammatory cytokines in macrophages and RGS4 in neurons
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-05 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.090, 33.740, 40.010 |
| Unit cell angles | 90.00, 92.70, 90.00 |
Refinement procedure
| Resolution | 31.590 - 1.960 |
| R-factor | 0.213 |
| Rwork | 0.209 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p1f |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.595 | 1.912 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.141 | 0.675 |
| Number of reflections | 9495 | |
| <I/σ(I)> | 10.8 | 1.2 |
| Completeness [%] | 98.2 | 76.5 |
| Redundancy | 5.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15-20% PEG 8000, 0.2M CaAcetate, 0.1 M cacodylate ph 6-7 |
