5CG9
NgTET1 in complex with 5mC DNA in space group P3221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-14 |
| Detector | RAYONIX MX300HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 191.216, 191.216, 51.254 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.742 - 2.693 |
| R-factor | 0.1942 |
| Rwork | 0.193 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lt5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.790 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.154 | 0.894 |
| Number of reflections | 29462 | |
| <I/σ(I)> | 13.7 | 2.2 |
| Completeness [%] | 98.7 | 92.8 |
| Redundancy | 9.3 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 20% (w/v) polyethylene glycol 8000, 200 mM Mg oxaloacetate, 100 mM Na cacodylate, pH 6.5 |
