5CG8
NgTET1 in complex with 5hmC DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 83.802, 107.360, 167.671 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.554 - 2.702 |
| R-factor | 0.1928 |
| Rwork | 0.191 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lt5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.892 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.600 | 2.790 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.066 | 0.977 |
| Number of reflections | 20985 | |
| <I/σ(I)> | 29.2 | 2.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9.9 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 25% (w/v) polyethylene glycol monomethyl ether 550, 10 mM ZnSO4, and 100 mM MES, pH 6.5 |
