5CFH
human beta-2 microglobulin double mutant W60G-Y63W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2014-03-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.346, 29.090, 44.410 |
| Unit cell angles | 90.00, 113.01, 90.00 |
Refinement procedure
| Resolution | 25.110 - 1.490 |
| R-factor | 0.17198 |
| Rwork | 0.169 |
| R-free | 0.22961 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z9t |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.752 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.110 | 1.570 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.075 | 0.220 |
| Number of reflections | 17435 | |
| <I/σ(I)> | 9.6 | 4.5 |
| Completeness [%] | 95.8 | 96.5 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 10000, HEPES |
