5CFE
Bacillus subtilis AP endonuclease ExoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-02-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.280, 61.920, 73.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.570 - 1.840 |
R-factor | 0.1691 |
Rwork | 0.167 |
R-free | 0.20760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bix |
RMSD bond length | 0.009 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.950 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.110 | 0.620 |
Number of reflections | 21097 | |
<I/σ(I)> | 12.09 | 2.99 |
Completeness [%] | 99.6 | 99.1 |
Redundancy | 6.3 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 16% (w/v) PEG 4000, 0.2 M CaCl2, 0.1 M Tris-HCl |