5CF9
Cleavage of nicotinamide adenine dinucleotide by the ribosome inactivating protein of Momordica charantia - enzyme-NADP+ co-crystallisation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.917 |
| Spacegroup name | H 3 |
| Unit cell lengths | 130.510, 130.510, 37.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.675 - 1.520 |
| R-factor | 0.1398 |
| Rwork | 0.138 |
| R-free | 0.16800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mom |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.500 | 1.560 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.058 | 0.910 |
| Number of reflections | 37103 | |
| <I/σ(I)> | 15.3 | 1.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | PEG 4000, sodium phosphate |
