5CB5
Structural Insights into the Mechanism of Escherichia coli Ymdb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 289.140, 289.140, 114.053 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.720 - 2.800 |
R-factor | 0.26296 |
Rwork | 0.262 |
R-free | 0.29036 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1spv |
RMSD bond length | 0.015 |
RMSD bond angle | 1.896 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.114 | 0.386 |
Number of reflections | 133057 | |
<I/σ(I)> | 12.7 | 3.1 |
Completeness [%] | 98.9 | 98.9 |
Redundancy | 3.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 285 | 2 M ammonium sulfate, 0.1 M BIS-TRIS |