5C9V
Structure of human Parkin G319A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91730 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 169.360, 169.360, 96.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.680 - 2.350 |
| R-factor | 0.1998 |
| Rwork | 0.198 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bm9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.691 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.680 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.089 | 0.800 |
| Number of reflections | 22270 | |
| <I/σ(I)> | 13.3 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 1.8 M lithium sulphate, 0.01 M MgCl2, 0.05 M MES pH 5.6 |
