5C9K
Crystal structure of a highly fibrillogenic Arg24Gly mutant of the Recombinant variable domain 6AJL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.556, 95.633, 102.583 |
| Unit cell angles | 90.00, 96.00, 90.00 |
Refinement procedure
| Resolution | 34.646 - 1.920 |
| R-factor | 0.1725 |
| Rwork | 0.170 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b5g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.265 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2083: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 2.000 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.073 | 0.460 |
| Number of reflections | 76561 | |
| <I/σ(I)> | 13.1 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | SOLUTION 7 FROM CRYSTAL REMARK 280 SCREEN I (HAMPTON RESEARCH), PH 6.5, VAPOR DIFFUSION, HANGING REMARK 280 DROP, TEMPERATURE 291K |
