5C7O
Structure of human testis-specific glyceraldehyde-3-phosphate dehydrogenase holo form with NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0746 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.442, 71.741, 80.844 |
| Unit cell angles | 90.00, 123.01, 90.00 |
Refinement procedure
| Resolution | 31.706 - 1.729 |
| R-factor | 0.138 |
| Rwork | 0.137 |
| R-free | 0.16640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h9e |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.710 |
| High resolution limit [Å] | 1.730 |
| Number of reflections | 68115 |
| <I/σ(I)> | 19.2 |
| Completeness [%] | 93.9 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 273 | 1:1 mixture of protein:NAD+ complex at 10 mg/mL with 0.2 M sodium nitrate, 20% PEG3350 |
