5C7N
Crystal structure of the human BRPF1 bromodomain in complex with Bromosporine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-20 |
| Detector | PSI PILATUS 2M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.502, 60.502, 63.111 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.310 - 1.750 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.310 |
| High resolution limit [Å] | 1.750 |
| Rmerge | 0.044 |
| Number of reflections | 13890 |
| <I/σ(I)> | 27.5 |
| Completeness [%] | 100.0 |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 20% PEG3350, 0.1 M sodium acetate, 0.15 M sodium nitrate, pH 5.5 |
