5C79
PH domain of ASAP1 in complex with diC4-PtdIns(4,5)P2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-07-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.543, 64.697, 44.428 |
Unit cell angles | 90.00, 95.63, 90.00 |
Refinement procedure
Resolution | 44.210 - 1.600 |
R-factor | 0.20018 |
Rwork | 0.198 |
R-free | 0.23345 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.889 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.600 |
Number of reflections | 27403 |
<I/σ(I)> | 15.3 |
Completeness [%] | 97.6 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1M HEPES, pH 7.5, 15% PEG 3350 |