5C65

Structure of the human glucose transporter GLUT3 / SLC2A3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	63.550, 116.030, 99.750
Unit cell angles	90.00, 106.91, 90.00

Refinement procedure

Resolution	40.000 - 2.650
R-factor	0.2062
Rwork	0.205
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pyp
RMSD bond length	0.009
RMSD bond angle	1.020
Data reduction software	xia2 (0.3.6.3)
Data scaling software	Aimless (0.5.9)
Phasing software	PHASER (2.5.6)
Refinement software	BUSTER (2.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.710	2.720
High resolution limit [Å]	2.650	2.650
Rmerge	0.088	1.139
Number of reflections	40188
<I/σ(I)>	16.8	1.4
Completeness [%]	99.8	99.2
Redundancy	7.9	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M citrate pH 5.5, 29% PEG400, 0.1M lithium sulfate