5C58
A double mutant of serratia marcescens hemophore receptor HasR in complex with its hemophore HasA and heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-06-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 101.971, 114.619, 260.757 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.749 - 2.795 |
| R-factor | 0.2496 |
| Rwork | 0.247 |
| R-free | 0.29640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3csl 1dk0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.667 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre-2083_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.960 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.095 | 2.227 |
| Number of reflections | 37882 | |
| <I/σ(I)> | 13.26 | 0.91 |
| Completeness [%] | 99.0 | 94.9 |
| Redundancy | 6.3 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100mM Tris pH 7 to 8, 1.8 to 2.2mM NaCl, 100mM K2HPO4 |
