5C3R
Crystal structure of the full-length Neurospora crassa T7H in complex with alpha-KG and 5-hydroxymethyluracil (5hmU)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.817, 155.775, 75.992 |
| Unit cell angles | 90.00, 91.65, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.350 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c3o |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.989 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
| Rmerge | 0.069 | 0.058 | 0.126 |
| Rmeas | 0.083 | 0.070 | 0.152 |
| Rpim | 0.046 | 0.039 | 0.084 |
| Total number of observations | 166072 | ||
| Number of reflections | 53781 | ||
| <I/σ(I)> | 15.3 | 10.2 | |
| Completeness [%] | 98.3 | 95.9 | 99.4 |
| Redundancy | 3.1 | 3 | 3.1 |
| CC(1/2) | 0.991 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M BIS-TRIS, 25%(W/v) PEG 3350 |
