5C2J

Complex structure of the GAP domain of MgcRacGAP and Cdc42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	43.324, 74.237, 55.423
Unit cell angles	90.00, 96.67, 90.00

Refinement procedure

Resolution	32.390 - 2.500
R-factor	0.198
Rwork	0.198
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ovj 1grn
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	12204
<I/σ(I)>	13.2	3.3
Completeness [%]	99.9	100
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	PEG3350, Bis-Tris