5C0Z
The structure of oxidized rat cytochrome c at 1.13 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.401, 52.471, 61.647 |
| Unit cell angles | 110.04, 92.77, 92.02 |
Refinement procedure
| Resolution | 49.220 - 1.124 |
| R-factor | 0.13321 |
| Rwork | 0.132 |
| R-free | 0.15928 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zoo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.903 |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.8.1) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.220 | 1.153 |
| High resolution limit [Å] | 1.124 | 1.124 |
| Rmerge | 0.050 | 0.548 |
| Number of reflections | 127840 | |
| <I/σ(I)> | 14.4 | 2 |
| Completeness [%] | 87.4 | 44.66 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 4000, isopropanol, sodium acetate, potassium ferricyanate |
