5BZ4

Crystal structure of a T1-like thiolase (CoA-complex) from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-08-06
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5
Spacegroup name	P 61
Unit cell lengths	190.076, 190.076, 265.084
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.519 - 2.430
R-factor	0.1641
Rwork	0.161
R-free	0.18750
RMSD bond length	0.009
RMSD bond angle	1.242
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.520
High resolution limit [Å]	2.430
Number of reflections	201832
<I/σ(I)>	8.2
Completeness [%]	98.9
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	293	45% PEG 200, 0.1M MES monohydrate pH 6.0, 0.07M calcium chloride dehydrate, 5mM CoA