5BWM

The complex structure of C3cer exoenzyme and GDP bound RhoA (NADH-bound state)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-19
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 32
Unit cell lengths	50.479, 50.479, 136.671
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.560 - 2.500
R-factor	0.21103
Rwork	0.209
R-free	0.24596
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1A2B and 3BW8
RMSD bond length	0.020
RMSD bond angle	1.737
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9)
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	13477
<I/σ(I)>	17.4	1.81
Completeness [%]	99.6	100
Redundancy	4.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM MES (pH 6.4), 20% PEG1500