5BW4

Crystal structure of the 16S rRNA (adenine(1408)-N(1))-methyltransferase W203A mutant with cosubstrate SAM from Catenulisporales acidiphilia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	65.718, 83.229, 93.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.310 - 2.100
R-factor	0.18618
Rwork	0.183
R-free	0.22957
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x1o
RMSD bond length	0.019
RMSD bond angle	2.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8.4-1496)
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.082
Number of reflections	30535
<I/σ(I)>	26.9	2.1
Completeness [%]	99.7	99.9
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	1.85 M Ammonium Sulfate, 0.08 M Tris, pH 8.5, 3.5% DMSO