5BVI

X-ray Structure of Interferon Regulatory Factor 4 IAD Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2013-10-18
Detector	PSI PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 21 2 21
Unit cell lengths	45.533, 84.854, 149.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.337 - 2.600
R-factor	0.1991
Rwork	0.194
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dsh
RMSD bond length	0.009
RMSD bond angle	1.145
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.360	2.360
Rmerge	0.153	0.660
Number of reflections	23107
<I/σ(I)>	8.7	2.1
Completeness [%]	93.4	89.1
Redundancy	4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	1.5-1.7M KCl, 0.1M Imidazole pH 8.0