5BRM

Structural basis for Mob1-dependent activation of the core Mst-Lats kinase cascade in Hippo signaling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	P 31 2 1
Unit cell lengths	142.188, 142.188, 135.981
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.295 - 2.651
R-factor	0.2452
Rwork	0.244
R-free	0.26810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pi1
RMSD bond length	0.006
RMSD bond angle	0.994
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.700
High resolution limit [Å]	2.650	7.180	2.650
Rmerge	0.091	0.058	1.000
Total number of observations	512365
Number of reflections	46680
<I/σ(I)>	11.3		1.8
Completeness [%]	99.5	97.8	97.9
Redundancy	11	10.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2.4 M Na Malonate