5BQD
Crystal Structure of TBX5 (1-239) Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2011-11-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 81.455, 81.455, 341.022 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.033 - 2.583 |
| R-factor | 0.2503 |
| Rwork | 0.246 |
| R-free | 0.28890 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.259 |
| Data reduction software | HKL-2000 (704k) |
| Data scaling software | HKL-2000 (704k) |
| Phasing software | PHENIX (1.9-1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.230 |
| High resolution limit [Å] | 2.583 |
| Number of reflections | 21874 |
| <I/σ(I)> | 8.88 |
| Completeness [%] | 92.1 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 100mM Sodium Cacodylate pH 6.4 10mM MgSO4 1.8M Ammonium Sulphate |
