5BPC
DNA polymerase beta ternary complex with a templating 5ClC and incoming dATP analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-30 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.654, 78.635, 55.077 |
| Unit cell angles | 90.00, 113.22, 90.00 |
Refinement procedure
| Resolution | 25.828 - 2.000 |
| R-factor | 0.2125 |
| Rwork | 0.210 |
| R-free | 0.25910 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.511 |
| Number of reflections | 54105 | |
| <I/σ(I)> | 17.6 | 2.407 |
| Completeness [%] | 98.9 | 90.9 |
| Redundancy | 4.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 50mM Imidazole, 350mM Sodium Acetate, 18% Peg3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |
