5BNQ
Crystal structure of hRANKL-mRANK complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 63 |
| Unit cell lengths | 122.188, 122.188, 94.473 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.370 - 2.800 |
| R-factor | 0.17246 |
| Rwork | 0.171 |
| R-free | 0.20292 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3me2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.303 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.197 | 0.818 |
| Number of reflections | 19916 | |
| <I/σ(I)> | 14.6 | 2.1 |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 12.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1 M sodium di-hydrogen phosphate, 2 M sodium chloride, 0.1 M potassium di-hydrogen phosphate and 0.1 M MES |
