5BMU

The crystal structure of the GST-like domains complex of AIMP3-EPRS mutant C92SC105SC123S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 31
Unit cell lengths	92.063, 92.063, 185.948
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.930 - 2.600
R-factor	0.13917
Rwork	0.138
R-free	0.16957
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bvx
RMSD bond length	0.011
RMSD bond angle	1.538
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	53953
<I/σ(I)>	16.5	2.8
Completeness [%]	100.0	100
Redundancy	5.4	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	293.15	Ammonium sulfate, Sodium chloride, Tris-HCl