5BJT
Crystal structure of human FcRn with a peptide inhibitor at multiple sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 104.917, 176.152, 245.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.270 - 3.200 |
| R-factor | 0.257 |
| Rwork | 0.252 |
| R-free | 0.33900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m17 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.965 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.111 | 0.718 |
| Number of reflections | 37736 | |
| <I/σ(I)> | 16.6 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 277 | 1.6 M ammonium sulfate, 20% glycerol, 0.8 M sodium acetate |
