5B8B
Crystal structure of reduced chimeric E.coli AhpC1-186-YFSKHN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.999999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.494, 135.654, 106.489 |
| Unit cell angles | 90.00, 111.22, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.25033 |
| Rwork | 0.249 |
| R-free | 0.28366 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n8j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.946 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 44808 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 92.9 | 92 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.6 M ammonium sulfate, 100 mM MES pH 6.5, 5% Dioxane |
