5B5K

Crystal structure of Izumo1, the mammalian sperm ligand for egg Juno

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98400
Spacegroup name	I 4 2 2
Unit cell lengths	103.280, 103.280, 139.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.840 - 2.500
R-factor	0.2577
Rwork	0.257
R-free	0.28070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jk9
RMSD bond length	0.004
RMSD bond angle	0.809
Data reduction software	XDS (20151015)
Data scaling software	XDS (20151015)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.840	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.195	0.230
Number of reflections	13389
<I/σ(I)>	12.13	1.13
Completeness [%]	100.0	100
Redundancy	12.6	10.1
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	0.2 M Ammonium formate pH 6.6, 20% PEG 3350