5B43

Crystal structure of Acidaminococcus sp. Cpf1 in complex with crRNA and target DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	81.515, 136.732, 196.909
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.155 - 2.800
R-factor	0.2221
Rwork	0.220
R-free	0.25710
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.447
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	196.910	2.880
High resolution limit [Å]	2.800	2.800
Number of reflections	54319
<I/σ(I)>	8.6
Completeness [%]	99.0
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	PEG3350, sodium acetate, 1,6-hexanediol