5B3J
Activation of NMDA receptors and the mechanism of inhibition by ifenprodil
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2014-04-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 247.249, 79.903, 181.314 |
Unit cell angles | 90.00, 127.09, 90.00 |
Refinement procedure
Resolution | 29.930 - 2.900 |
R-factor | 0.275 |
Rwork | 0.273 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.068 |
Data reduction software | HKL-2000 |
Data scaling software | PHENIX |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.099 |
Number of reflections | 57592 |
<I/σ(I)> | 8.5 |
Completeness [%] | 91.4 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 0.1M sodium acetate, 27% PEG3350, 2.2M sodium formate, 0.05 M calcium chloride |